Modern Care Journal

Published by: Kowsar

Genotyping of Cystic Hydatidosis Agents in Birjand, Eastern Iran

Mehdi Karamian 1 , * , Fatemeh Haghighi 2 and Mina Hemmati 3
Authors Information
1 Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
2 Department of Pathology, Birjand University of Medical Sciences, Birjand, Iran
3 Birjand Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
Article information
  • Modern Care Journal: July 2017, 14 (3); e64531
  • Published Online: April 30, 2017
  • Article Type: Research Article
  • Received: January 31, 2017
  • Revised: February 14, 2017
  • Accepted: April 1, 2017
  • DOI: 10.5812/modernc.64531

To Cite: Karamian M, Haghighi F, Hemmati M. Genotyping of Cystic Hydatidosis Agents in Birjand, Eastern Iran, Mod Care J. 2017 ;14(3):e64531. doi: 10.5812/modernc.64531.

Copyright: Copyright © 2017, Modern Care Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Methods
3. Results
4. Discussion
  • 1. Sadjjadi SM. Present situation of echinococcosis in the Middle East and Arabic North Africa. Parasitol Int. 2006;55 Suppl:S197-202. doi: 10.1016/j.parint.2005.11.030. [PubMed: 16337429].
  • 2. Zhang T, Yang D, Zeng Z, Zhao W, Liu A, Piao D, et al. Genetic characterization of human-derived hydatid cysts of Echinococcus granulosus sensu lato in Heilongjiang Province and the first report of G7 genotype of E. canadensis in humans in China. PLoS One. 2014;9(10). e109059. doi: 10.1371/journal.pone.0109059. [PubMed: 25329820].
  • 3. Nakao M, Lavikainen A, Yanagida T, Ito A. Phylogenetic systematics of the genus Echinococcus (Cestoda: Taeniidae). Int J Parasitol. 2013;43(12-13):1017-29. doi: 10.1016/j.ijpara.2013.06.002. [PubMed: 23872521].
  • 4. Schneider R, Gollackner B, Edel B, Schmid K, Wrba F, Tucek G, et al. Development of a new PCR protocol for the detection of species and genotypes (strains) of Echinococcus in formalin-fixed, paraffin-embedded tissues. Int J Parasitol. 2008;38(8-9):1065-71. doi: 10.1016/j.ijpara.2007.11.008. [PubMed: 18177654].
  • 5. Sarkari B, Rezaei Z. Immunodiagnosis of human hydatid disease: Where do we stand?. World J Methodol. 2015;5(4):185-95. doi: 10.5662/wjm.v5.i4.185. [PubMed: 26713278].
  • 6. Lotsch F, Naderer J, Skuhala T, Groger M, Auer H, Kaczirek K, et al. Intra-cystic concentrations of albendazole-sulphoxide in human cystic echinococcosis: a systematic review and analysis of individual patient data. Parasitol Res. 2016;115(8):2995-3001. doi: 10.1007/s00436-016-5054-x. [PubMed: 27085708].
  • 7. Hemphill A, Stadelmann B, Rufener R, Spiliotis M, Boubaker G, Muller J, et al. Treatment of echinococcosis: albendazole and mebendazole--what else?. Parasite. 2014;21:70. doi: 10.1051/parasite/2014073. [PubMed: 25526545].
  • 8. Buttenschoen K, Carli Buttenschoen D. Echinococcus granulosus infection: the challenge of surgical treatment. Langenbecks Arch Surg. 2003;388(4):218-30. doi: 10.1007/s00423-003-0397-z. [PubMed: 12845535].
  • 9. Maravilla P, Andrew Thompson RC, Palacios-Ruiz JA, Estcourt A, Ramirez-Solis E, Mondragon-de-la-Pena C, et al. Echinococcus granulosus cattle strain identification in an autochthonous case of cystic echinococcosis in central Mexico. Acta Trop. 2004;92(3):231-6. doi: 10.1016/j.actatropica.2004.07.001. [PubMed: 15533292].
  • 10. Sharbatkhori M, Mirhendi H, Harandi MF, Rezaeian M, Mohebali M, Eshraghian M, et al. Echinococcus granulosus genotypes in livestock of Iran indicating high frequency of G1 genotype in camels. Exp Parasitol. 2010;124(4):373-9. doi: 10.1016/j.exppara.2009.11.020. [PubMed: 20004194].
  • 11. Varcasia A, Canu S, Kogkos A, Pipia AP, Scala A, Garippa G, et al. Molecular characterization of Echinococcus granulosus in sheep and goats of Peloponnesus, Greece. Parasitol Res. 2007;101(4):1135-9. doi: 10.1007/s00436-007-0568-x. [PubMed: 17484070].
  • 12. Dinkel A, Njoroge EM, Zimmermann A, Walz M, Zeyhle E, Elmahdi IE, et al. A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa. Int J Parasitol. 2004;34(5):645-53. doi: 10.1016/j.ijpara.2003.12.013. [PubMed: 15064129].
  • 13. Gudewar J, Pan D, Bera AK, Das SK, Konar A, Rao JR, et al. Molecular characterization of Echinococcus granulosus of Indian animal isolates on the basis of nuclear and mitochondrial genotype. Mol Biol Rep. 2009;36(6):1381-5. doi: 10.1007/s11033-008-9324-2. [PubMed: 18668343].
  • 14. Sanchez E, Caceres O, Naquira C, Miranda E, Samudio F, Fernandes O. Echinococcus granulosus genotypes circulating in alpacas (Lama pacos) and pigs (Sus scrofa) from an endemic region in Peru. Mem Inst Oswaldo Cruz. 2012;107(2):275-8. [PubMed: 22415269].
  • 15. Casulli A, Zeyhle E, Brunetti E, Pozio E, Meroni V, Genco F, et al. Molecular evidence of the camel strain (G6 genotype) of Echinococcus granulosus in humans from Turkana, Kenya. Trans R Soc Trop Med Hyg. 2010;104(1):29-32. doi: 10.1016/j.trstmh.2009.08.001. [PubMed: 19786289].
  • 16. Zhang L, Eslami A, Hosseini SH, McManus DP. Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mitochondrial DNA markers. Am J Trop Med Hyg. 1998;59(1):171-4. [PubMed: 9684648].
  • 17. Sharbatkhori M, Mirhendi H, Jex AR, Pangasa A, Campbell BE, Kia EB, et al. Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning-phylogenetic approach. Electrophoresis. 2009;30(15):2648-55. doi: 10.1002/elps.200900145. [PubMed: 19637222].
  • 18. Farhadi M, Fazaeli A, Haniloo A. Genetic characterization of livestock and human hydatid cyst isolates from northwest Iran, using the mitochondrial cox1 gene sequence. Parasitol Res. 2015;114(12):4363-70. doi: 10.1007/s00436-015-4673-y. [PubMed: 26280086].
  • 19. Rostami S, Shariat Torbaghan S, Dabiri S, Babaei Z, Ali Mohammadi M, Sharbatkhori M, et al. Genetic characterization of Echinococcus granulosus from a large number of formalin-fixed, paraffin-embedded tissue samples of human isolates in Iran. Am J Trop Med Hyg. 2015;92(3):588-94. doi: 10.4269/ajtmh.14-0585. [PubMed: 25535316].
  • 20. Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992;54(2):165-73. [PubMed: 1435857].
  • 21. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013;30(12):2725-9. doi: 10.1093/molbev/mst197. [PubMed: 24132122].
  • 22. Fasihi Harandi M, Budke CM, Rostami S. The monetary burden of cystic echinococcosis in Iran. PLoS Negl Trop Dis. 2012;6(11). e1915. doi: 10.1371/journal.pntd.0001915. [PubMed: 23209857].
  • 23. Guarnera EA, Parra A, Kamenetzky L, Garcia G, Gutierrez A. Cystic echinococcosis in Argentina: evolution of metacestode and clinical expression in various Echinococcus granulosus strains. Acta Trop. 2004;92(2):153-9. doi: 10.1016/j.actatropica.2004.06.008. [PubMed: 15350868].
  • 24. Sharma M, Sehgal R, Fomda BA, Malhotra A, Malla N. Molecular characterization of Echinococcus granulosus cysts in north Indian patients: identification of G1, G3, G5 and G6 genotypes. PLoS Negl Trop Dis. 2013;7(6). e2262. doi: 10.1371/journal.pntd.0002262. [PubMed: 23785531].
  • 25. McManus DP, Zhang L, Castrodale LJ, Le TH, Pearson M, Blair D. Short report: molecular genetic characterization of an unusually severe case of hydatid disease in Alaska caused by the cervid strain of Echinococcus granulosus. Am J Trop Med Hyg. 2002;67(3):296-8. [PubMed: 12408670].
  • 26. Romig T, Ebi D, Wassermann M. Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato. Vet Parasitol. 2015;213(3-4):76-84. doi: 10.1016/j.vetpar.2015.07.035. [PubMed: 26264250].
  • 27. Amer S, Helal IB, Kamau E, Feng Y, Xiao L. Molecular characterization of Echinococcus granulosus sensu lato from farm animals in Egypt. PLoS One. 2015;10(3). e0118509. doi: 10.1371/journal.pone.0118509. [PubMed: 25760944].
  • 28. Ebadzadeh H, Ahmadi K, Mohammadnia-Afrozi S, Abbas-Taghani R, Moradi-Eslami A, Abbasi M. Agricultural statistics [In Persian]. 2. Tehran: Ministry of agriculture; 2016. 384 p.
  • 29. Konyaev SV, Yanagida T, Ingovatova GM, Shoikhet YN, Nakao M, Sako Y, et al. Molecular identification of human echinococcosis in the Altai region of Russia. Parasitol Int. 2012;61(4):711-4. doi: 10.1016/j.parint.2012.05.009. [PubMed: 22609955].
  • 30. Sadjjadi SM, Mikaeili F, Karamian M, Maraghi S, Sadjjadi FS, Shariat-Torbaghan S, et al. Evidence that the Echinococcus granulosus G6 genotype has an affinity for the brain in humans. Int J Parasitol. 2013;43(11):875-7. doi: 10.1016/j.ijpara.2013.06.008. [PubMed: 23891711].
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